As a CELLutions SUMMIT delegate you're invited to
attend a Hands-On Workshop organized by the Tissue
Engineering Resource Center (TERC) at Tufts University.
DAY 1
Sunday, August 19
8-8:30AM – Registration/breakfast
8:30-9:30 – Background/Introductory
lecture/safety
9:30-11:30 – Exploring Stem Cells – (Choose 1)
Introductory lecture
Cell culture (expansion, differentiation media, FACS,
maintenance media, scale-
up, counting, storage, cell-specific assays,
transformation/transfection, RT-PCR quantification)
Option 1
à Adipose Stem Cells
Option 2 à Peri-natal
Stem Cells
Option 3 à Mesenchymal
Stem Cells
Option 4 à Embryonic
Stem Cells
11:30-12:30 – Think Tank: Facilitated by morning
group leaders; mini groups will recombine for an
opportunity to ask questions on alternate cell types,
troubleshoot laboratory concerns, discuss and
brainstorm.
12:30-1:00 PM – Lunch
1:00-5:00 PM – (Choose 1)
Module 1: Imaging -
Sample preparation and basic techniques tailored to
imaging stem cells with:
- Scanning Electron
Microscopy
- Multiphoton/Confocal
Microscopy
- UV- and
inverted-fluorescence Microscopy
- Atomic Force Microscopy
Module 2: Continuous and Co-cultures –
demonstration and review of bioreactor systems to
improve in vitro culture conditions, promote
stemcell viability and differentiation.
- Spinner flasks and rotating
vessels
- Multi-well shear devices
- Round perfusion reactors
- Removable rod reactors
- Bioreactors with electrical
stimulation
- Reactor design and modeling
Co-culture techniques with stem cells, as well as
incorporation with bioreactors to grow tissues in
vitro will be addressed.
5:00 PM – Wrap up of day one
DAY 2
Monday, August 20
9 AM- Breakfast
9:00 – 12:00 AM – Sessions
Expert "show and tell" - Putting stem cells to work
Topics
Immunofluorescence and 3D skin models
Disease models
Cardiac tissue engineering
Lentiviral transfection systems
Stem cells from cord and placenta tissue
Microfluidic systems and cell migration
Legalities of stem cell research
*Session topics subject to change and/or expansion
to cover other aspects of current stem cell
technologies.
12:00-1:00 – Lunch
1:00 – 4:00 PM – Sessions as above in a second
topic of choice
Note - Limited space is available. All payments must
be paid in full to process your registration. For
further information about TERC please visit
www.ase.tufts.edu/terc
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