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CONFERENCE SERIES: Biological Therapeutic Products

Recorded at: PepTalk: The Protein Science Week

Digital Course: Protein Crystallization - Delineating Protein Structure

 

Order DVDDVD Cover About this Product:
The “Protein Crystallization” digital course provides both an overview of crystallizing proteins as well as an introduction to crystallography.  The particular challenges of crystallizing Membrane Proteins are addressed, and innovations for automating protein crystallization are included.  This is a perfect introduction to Protein Scientists who want to learn more about how proteins are crystallized, and where the technology is headed.

 


About this Product:
2 Presentations
Over 50 Slides
104 Minutes
Individual Copy: $345
Site License: $1380

Agenda At A Glance:
Crystallography is a widely recognized tool that supports protein science. For Membrane Proteins, this tool is elevated into an important science that aids in the efforts to reveal structures and functions. This course describes how crystallography works, and includes highlights of Membrane Proteins and automation.

Speakers 

Introduction to Protein Crystallization
Peter Nollert Peter Nollert, Ph.D., Director, Membrane Protein Technologies, Emerald BioStructures, Inc.
The topic of protein crystallization is introduced with an emphasis on current best practices in the field.
The following facets will be explored in more detail: protein sample quality, crystallization methods and tools, primary screening and optimization.  Finally, the preparation of protein crystals for X-ray diffraction experiments and the use of protein crystals in drug discovery will be introduced.

Biography: Dr. Nollert received his doctorate (Dr. rer. Nat.) in Biochemistry from the Eberhard-Karls-Universität Tübingen, and titled his thesis “Immobilization of Membranes on Solid Surfaces.”  Subsequently he had two postdocs, first at Biozentrum Basel working with Membrane Protein Crystallization in Lipidic Cubic Phases, and then at Stanford University and UCSF.  Dr. Nollert maintains a number of blogs on membrane proteins, and his research interests include:  membrane protein crystallization, method development, life science, technology innovation, GPCRs, structural biology, and X-ray crystallography.

Automated High-Throughput Crystallization of Membrane Proteins in Lipidic Cubic Phase
Frank Schafer Frank Schäfer, Ph.D., Associate Director, Head of DNA & Protein Sciences, Research & Development, QIAGEN GmbH
Crystallization of membrane proteins is one of the most demanding biological applications to date, which is reflected by the small number of membrane protein structures disposed in the protein database. On the other hand, 30% of all known proteins are located in the membrane, and many of them are druggable targets (like G protein coupled receptors). Therefore, the importance of membrane protein crystallization for structure-based drug design cannot be overestimated.

We present CubicPhase, a new protein crystallization method based on vapor diffusion for high-throughput, automated crystallization setup of membrane proteins using standard nanoliter dispensing robots and ready-to-use micro plates pre-coated with lipid. Crystallization of the membrane proteins occurs in meso using Lipidic Cubic Phase (LCP) technology. New screening suites have been developed for in meso applications.

We will provide data on crystallization of several human and bacterial membrane proteins and highlight the advantages of the combination of in meso crystallization with vapor diffusion, experimental flexibility, and automation. Several of the features of our LCP technology will be demonstrated in a time lapse movie showing the formation of crystals of a colored membrane protein in a real experiment.  The structure of a novel 7-transmembrane domain protein, Sensory Rhodopsin II, has been solved at 2.7 Å resolution using CubicPhase and will be presented in brief.

Biography: Dr. Schäfer studied Microbiology at the Philipps University of Marburg, Germany, and obtained his Ph.D. in Molecular Biology and Biochemistry from the Institute of Molecular Biology and Tumor Research at the University of Marburg. His studies included work on the regulation of transcription in eukaryotes.

Dr. Schäfer has been in Research & Development for over 14 years. His current responsibilities at QIAGEN include the development of Protein Science products for recombinant protein expression, purification and crystallization, native protein sample preparation, and protein assay development, as well as development of its plasmid DNA and DNA purification products.

About the Conference:
CHI’s 10th Annual PepTalk event features strong scientific programming comprising fourteen conferences within topic focused pipelines. The four distinct pipelines range from applying protein discovery research, to developing downstream protein expression, characterization, formulation, and production that ultimately leads to clinical applications. This event is designed with you in mind. Stay within a specific pipeline or track hop, and create a custom agenda to meet your research and networking needs.