Detailed analytics of any process encourages improvements.   Pipeline 2 weaves the thread of the protein production pipeline from beginning to end and back again.  The circular process of evaluating analytical data continually refines the required steps and provides a guide for optimization, thus enhancing quality and reducing bottom-line costs.  The decisions made along this process enable productivity, consistency and product/protein quality.   Computational modeling, a new tool in the production pipeline, further enhances these strategic decisions.