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Protein Expression: Overcoming Challenges header

Despite decades of research and advances, some areas of protein science remain extremely challenging and complex.  Antibodies, vaccines, human proteins, and other difficult-to-express proteins have fueled new research and expression methodologies and technologies.  High throughput purification and tags promise great rewards, but still pose important questions for researchers.  Cell free expression methods hold great potential, but pose new challenges.  This meeting addresses the newest developments in these protein science fields and provides insights and solutions for conquering these issues.

OPTIMIZING EXPRESSION

Performance by Design: Engineering Functionality into Biopharmaceutical Products
Susan Dana Jones, Ph.D., Vice President and Senior Consultant, BioProcess Technology Consultants, Inc.

Engineering the Salmonella Type III Secretion System to Export Spider Silk Monomers
Daniel Widmaier, Researcher, Voigt Lab, Chemistry and Chemical Biology Graduate Program, University of California, San Francisco

 

TAGS AND PURIFICATION

The Use of SUMO-Tag to Improve Protein Expression and Factors Affecting Its Removal
Bingyuan Wu, Ph.D., Research Scientist, Biologics Research, Centocor R & D

 

 

PLENARY SESSION – EXTRACTION, EXPRESSION & ACHIEVING SCALE WITH MEMBRANE PROTEINS

Detergents in the Purification, Stabilization, and Refolding of Membrane Proteins
Dirk Linke, Ph.D., Professor, Protein Evolution, Max Planck Institute for Developmental Biology

Preparation of Stable Isotope-Labeled GPCR by Bacterial Fermentation
Alexei Yeliseev, Ph.D., Staff Scientist, Laboratory of Membrane Biochemistry and Biophysics, NIH/NIAAA

Scale Up and Process Development of a Suite of Membrane Bound Proteins (CYP450s) in Saccharomyces cerevisiae
Prabuddha Kundu, Ph.D., VP, Manufacturing, Premas Biotech Pvt. Ltd.

 

Breakfast Presentation

DNA 2.0


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