Great strides have been made in the expression of proteins for biotherapeutics; however, with these strides have come new hurdles. Higher throughput expression and purification, as well as more flexible expression systems and techniques are in even greater demand now to support the needs of the research pipeline. This meeting explores the newest data and innovations relating to the expression and characterization of proteins for biological understanding and therapeutics, as well as strategies to make the eventual expression of these proteins more effective, efficient and trouble-free. Attention is given to new hosts and platforms, as well as how to select, engineer and optimize these hosts.
Opening Keynote Presentation
Significant Enhanced Expression and Solubility of Human Proteins in Escherichia coli by Fusion with Protein S from Myxococcus xanthus
Masayori Inouye, Ph.D., Department of Biochemistry, Robert Wood Johnson Medical School
Structural Insights into Bacteriophage Protein Complexes Revealed by Hybrid Methods
David Veesler, Researcher, Architecture et Fonction des Macromolécules Biologiques, Université de Provence, Université de la Méditerranée
Secretion and Proteolysis of Heterologous Proteins Fused to the Escherichia coli Maltose Binding Protein in Pichia pastoris
Geoffrey Lin-Cereghino, Ph.D., Department of Chemistry, University of the Pacific
Overcoming Bottle-Neck Problems in Producing Recombinant Therapeutic Proteins at Early Stages and the Potential Impact of Novel Conformases/Foldases on the Field
M. Raafat El-Gewely, Ph.D., Institute of Medical Biology, University of Tromsø
Fusion Partners as a Tool for the Expression of Difficult Proteins in Mammalian Cells
Christopher Mehlin, Ph.D., Department of Protein Science, Amgen, Inc.
Strategies for the Enhancement of Recombinant Protein Production from Mammalian Cells by Growth Arrest
Kevin Sunley, Ph.D., Post-Doctoral Researcher, Department of Microbiology, University of Manitoba
Expression and Purification of the Membrane Protein p7 from HCV
Stanley J. Opella, Ph.D., Department of Chemistry and Biochemistry, University of California, San Diego
Quality Optimization of Cell-Free Expressed Membrane Proteins: Characterization of the Human Endothelin Receptom
Frank Bernhard, Ph.D., Lab Leader, Institute of Biophysical Chemistry, Goethe University, Frankfurt
When Proteins Misbehave, Try a Little Pressure: Refolding Polnu
Robert M. Petrovich, Ph.D., Head, Protein Expression Core Facility, Laboratory of Structural Biology, NIEHS, National Institutes of Health
Development of a Fully Automated, Miniaturized Bioreactor System for Optimization of Protein Production Processes
Jacques Bellalou, Ph.D., Head of Platform for Recombinant Protein Production in Microorganisms, Structural Biology and Chemistry Dept., Institut Pasteur
High-Throughput Protein Expression Using an Improved Drosophila S2 Expression System
Wian de Jongh, Ph.D., Team Leader, Insect Cell Expression, Protein Production Unit, The Novo Nordisk Foundation, Center for Protein Research
A Toolbox for High-Throughput Parallel Clone Construction for Protein Expression Optimization in Multiple Host Systems
Dominic Esposito, Ph.D., Principal Scientist, Protein Expression Laboratory, SAIC-Frederick, Inc.