The demand for high quality biotherapeutic proteins has never been greater, yet meeting this demand is challenging because protein expression is both an art and a science. Bottlenecks frequently arise because functional proteins are difficult to produce. This usually requires designing new cloning schemes including lengthy verification and sequence analysis of the gene or protein of interest, moving a gene from one vector to another, transfecting the vector in an alternative host, or re-characterizing the expressed protein --an inefficient, time-consuming and expensive process. This meeting continues the tradition of applying effective protein discovery research leading to functional products.
Variants of a Novel Bipartite Plant Viral Vector System Adapted for High Level Protein Expression or Virus-Induced Gene Silencing
John Hammond, Ph.D., Research Plant Pathologist, Floral and Nursery Plants Research Unit, USDA-ARS
A Suite of Biosafe Deleted Plant Viral Vectors for High Level Protein Expression
Christopher Kearney, Ph.D., Associate Professor, Biology, Baylor University
Use of Lentiviral Vector Promoter Libraries to Optimize Protein Expression in Mammalian Cell Lines
Dominic Esposito, Ph.D., Principal Scientist, Protein Expression Laboratory, SAIC-Frederick, Inc.
Modulation of Plasmid Replication for Optimal Expression of Recombinant Genes
Manel Camps, Ph.D., Assistant Professor, Microbiology and Environmental Toxicology, University of California, Santa Cruz
Independently Inducible System of Gene Expression for Condensed Single Protein Production (cSPP) Suitable for High Efficiency Isotope Enrichment
Monica Roth, Ph.D., Professor, Biochemistry, University of Medicine and Dentistry, New Jersey
Recombinant Yeast Libraries as a Tool for Engineering Novel Antibacterial Proteins
Karl E. Griswold, Ph.D., Assistant Professor, Thayer School of Engineering, Dartmouth College
Protein Structure Initiative Material Repository: An Open Shared Public Resource of Structural Genomics Plasmids for the Biological Community
Jason Steel, Ph.D., Research Scientist, Center for Personalized Diagnostics, Arizona State University
Single Gene Copy CHO Production Lines: The EESYR Approach to High-Level Protein Expression for Therapeutic Protein Development
Anke Watty, Ph.D., Staff Scientist, Protein Expression Sciences, Regeneron Pharmaceuticals
Production of Recombinant Proteins from Protozoan Parasites: Current Approaches and Novel Platforms
José-Antonio Fernández-Robledo, Ph.D., Assistant Professor, Microbiology and Immunology, University of Maryland School of Medicine, IMET
Interplay between Translation Rates and Protein Folding Efficiency
José M. Barral, M.D., Ph.D., Assistant Professor, Neuroscience & Cell Biology, The University of Texas Medical Branch
Intraclonal Protein Expression Heterogeneity in Recombinant CHO Cells
Peter Gray, Ph.D., Director, Australian Institute for Bioengineering and Nanotechnology (AIBN), The University of Queensland