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Cambridge Healthtech Institute’s 2nd Annual
Transient Protein Production
From Small-Scale to Large-Scale
January 22-23, 2015

Speed, limiting risk and protein quality are often cited as advantages of transient protein production (TPP). Transient expression achieves both fast process times and high yields because the gene of interest is simply transfected via a plasmid into the respective producer cells. In addition, compared to expression in lower-order organisms, recombinant protein expression in mammalian producer cells (CHO and HEK293) or alternative hosts offers significant advantages in protein quality and posttranslational modification. However, the rapidly increasing need for recombinant proteins necessitates further improvements in TPP technologies.

Cambridge Healthtech Institute’s Second Annual Transient Protein Production conference convenes protein expression specialists who share their experiences of the differences, tradeoffs, advantages and improvements in producing recombinant proteins in transient production systems.

Preliminary Agenda 


ENGINEERING HIGHER TRANSIENT EXPRESSION
IN MAMMALIAN SYSTEMS

Assay Cell Line Development

Jennitte Stevens, Ph.D., Principal Scientist, Amgen, Inc.

Toolbox to Streamline the Identification of Antibody Drug Candidates

Giovanni Magistrelli, Engineer, Head, Protein Generation, Novimmune

Enhancement of Transient Protein Expression via Modulation of Intracellular Biomarkers and Discovery of Novel Non-Viral Delivery Vehicles

Kaushal Rege, Ph.D, Associate Professor, Chemical Engineering; Faculty, Biomedical Engineering and Biological Design, Arizona State University

Anti-Inflammatory Tristetraprlin Expression in HEK293 Cells

Heping Cao, Ph.D., Principal Research Scientist, Southern Regional Research Center, U.S. Department of Agriculture

MaxCyteRapid Protein Production with Flow Electroporation: Higher Titers, Cell Type Flexibility, and Scalability

James Brady, Ph.D., MBA, Director, Technical Applications, MaxCyte, Inc.


ENGINEERING HIGHER TRANSIENT EXPRESSION IN ALTERNATIVE HOSTS

Glycoengineering in the Baculovirus-Insect Cell System

Don Jarvis, Ph.D., Professor, Molecular Biology, University of Wyoming

Modifications to the Baculovirus/Insect Cell Expression System to Enhance Production of Prenylated Proteins

Dominic Esposito, Ph.D., Director, Protein Expression Laboratory, Frederick National Laboratory for Cancer Research, Leidos Biomedical Research, Inc.

High-Level Transient Expression of Protein Biologics in Plants through Agroinfiltration

Qiang (Shawn) Chen, Ph.D., Associate Professor, The Biodesign Institute and School of Life Sciences, Arizona State University


ESTABLISHING A FLEXIBLE PROTEIN PRODUCTION FACILITY

The Speed and Flexibility of Scalable Transient Protein Production: Meeting the Demands for a Wide Variety of Early Discovery Studies and Applications

Richard Altman, MS, Research Scientist, Molecular Sciences, Alexion Pharmaceuticals

Nimble Protein Production from Mammalian Cells in a Mid-Size Research Setting

Thomas Cameron, Ph.D., Associate Director, Biologics Drug Discovery, Research Division, Biogen Idec