TUESDAY, JANUARY 15 | 5:45 - 8:45 PM
SC5: TRANSIENT PROTEIN PRODUCTION IN MAMMALIAN CELLS
Room: Aqua 311
Instructors:
Richard Altman, MS, Scientist, Protein Technologies, Amgen
Henry C. Chiou, PhD, Director, Cell Biology, Life Science Solutions, Thermo Fisher Scientific
Bojiao Yin, PhD, Scientist, Protein Technologies, Amgen
A variety of mature protein production systems exist that can be used to create an expression toolbox to address protein production challenges. This short course focuses on transient protein production in mammalian cells including the concepts, technologies, and optimization strategies needed for the rapid generation of milligram-to-gram quantities of secreted or intracellular recombinant proteins for therapeutic, functional, and structural studies. The course combines instruction and case studies in an interactive environment directed towards intermediate-level scientists, but is still appropriate for expression scientists of all experience levels.
Fundamental Elements of Transient Protein Production
- Expression vectors for protein production
- HEK293 vs. CHO
- The interplay of cells, medium, transfection reagent, and supplements
- Key considerations for maximizing yields
BacMam Application in Protein Expression
- Transient transfection using BacMam vector
- Optimization for BacMam production
Implementing Transient Protein Production
- Assembling an effective mammalian expression toolbox
- Optimizing the total protein expression process
Instructor Biographies:
Richard Altman, MS, Scientist, Protein Technologies, Amgen
Rich Altman has 30 years of experience working in the pharmaceutical industry. In early 2016, he joined the Protein Technologies Mammalian Expression group at Amgen San Francisco, supporting biologics drug development. Prior to Amgen, he worked for several pharmaceutical companies on the cloning, expression, purification and characterization of recombinant proteins. This work supported both small-molecule high-throughput screening and protein therapeutic efforts. He received his MS degree from the University of Pittsburgh School of Medicine in the Department of Molecular Biology and Biochemistry.
Henry C. Chiou, PhD, Director, Cell Biology, Life Science Solutions, Thermo Fisher Scientific
Dr. Chiou has over 15 years of experience working on development of mammalian protein expression systems and transfection reagents. For the past nine years, he has worked as a principal scientist and now in product development and portfolio management for Life Technologies, now part of Thermo Fisher Scientific. He has led the development efforts on a number of products, including the FreeStyle™ transient expression systems, Lipofectamine™ LTX and Lipofectamine™ RNAiMAX. Prior to Life Technologies, he worked for several biotech companies on biotherapeutic gene delivery systems. Dr. Chiou received his doctorate from Harvard University and completed a postdoctoral fellowship at the University of Pennsylvania.
Bojiao Yin, PhD, Scientist, Protein Technologies, Amgen
Dr. Yin has over 8 years of experience working in mammalian cell culture and focusing on novel platform development. She worked at the Protein Technologies Mammalian Expression group at Amgen Massachusetts, supporting both large molecule and small molecule drug development. Prior to Amgen, she completed a postdoctoral fellowship in Dr. Michael J Betenbaugh's lab at Johns Hopkins University. She focused on cell line engineering, media supplement development and omics analysis in CHO cells. Dr. Yin did her doctorate at Chinese Academy of Sciences (Beijing).