In biologic discovery, purification strategies must be tailored to varying scales and business needs, especially when considering higher throughput workflows. This talk outlines core chromatography principles, compares batch versus column approaches, and evaluates tip-based, pre-packed, and other innovative techniques needed to automate these processes. Emphasizing time, cost, binding/elution efficiency, and ease automation, the study helps make fit-for-purpose choices that close industry gaps and strengthen end-to-end biologic discovery workflows.

Transcription factors and other intracellular protein-protein interactions remain challenging therapeutic targets. This presentation describes an integrated live-cell discovery platform combining genetically encoded peptide libraries, intracellular cyclisation, functional selection and scalable hit validation. By constraining peptides during selection, the workflow enriches cell-tolerated, biostable antagonists that disrupt transcription factor-DNA and protein-protein interactions. The approach links library design, screening, sequencing-based prioritization, biophysical characterization and cellular validation to accelerate discovery of functional, cell-active constrained peptides.

G-protein-coupled receptors (GPCRs) have key roles in physiology and are central targets for drug discovery and development, but the design of protein agonists and antagonists has been challenging as GPCRs are conformationally dynamic integral membrane proteins. Skape Bio will present de novo design methods and a high-throughput microscopy-based screen coupled to semi-automated design-built-test-learn cycles for generation of GPCR-modulating miniproteins with high affinity, potency and selectivity.