Cambridge Healthtech Institute’s 28th Annual
Recombinant Protein Production - Part 1
January 19-20, 2027
Cambridge Healthtech Institute’s 28th Annual Recombinant Protein Production – Part 1 conference focuses on practical strategies for producing target proteins that remain persistently difficult to express, fold, and stabilize. Despite decades of progress in recombinant protein production, many target classes continue to challenge even the most experienced teams, including GPCRs, ion channels, transporters, intrinsically disordered proteins, proteases, PTM-sensitive proteins, disulfide-rich proteins, de novo binders, AI-designed proteins, and miniprotein targets. This year’s program examines how construct design, cassette architecture, culture conditions, and expression strategy can be optimized to improve yield while preserving functional activity and protein quality. The agenda will showcase bench-tested approaches for troubleshooting low expression, misfolding, instability, aggregation, and poor recovery. Join us to explore practical solutions that accelerate the production of challenging recombinant protein targets and help move difficult proteins from design to usable material.
Coverage will include, but is not limited to:
PRODUCING GPCRs AND MEMBRANE PROTEINS
- GPCRs for screening and structural biology
- Ion channels and transporters
- Preserving native conformation
INTRINSICALLY DISORDERED PROTEINS, PROTEASES, AND PROTEINS WITH DIFFICULT PTMs
- Intrinsically disordered proteins
- Hydrophobic and aggregation-prone targets
- Proteins with unstable cores
- PTM-sensitive targets
- Proteases and other difficult-to-express enzyme targets
- Disulfide-rich proteins
EXPRESSING DE NOVO, AI-DESIGNED, AND MINIPROTEIN TARGETS
- Troubleshooting low expression and misfolding
- Producing miniproteins, minibinders, and compact binding domains
- Expression strategies for AI-designed proteins, de novo binders, and synthetic scaffold
CONSTRUCT, CASSETTE, AND CULTURE DESIGN FOR TARGET EXPRESSION
- Codon usage, promoters, signal peptides, tags, and vector architecture
- Media, temperature, induction, feeding, and culture-condition strategies for improving expression
- AI/ML tools for predicting expression level, solubility, secretion, etc.
The deadline for priority consideration is June 26, 2026.
All proposals are subject to review by session chairpersons and/or the Scientific Advisory Committee to ensure the overall quality of the conference program. Additionally, as per Cambridge Healthtech Institute’s policy, a select number of vendors and consultants who provide products and services will be offered opportunities for podium presentation slots based on a variety of Corporate Sponsorships.
Opportunities for Participation: