Shishir P. S. Chundawat, PhD, Associate Professor, Department of Chemical and Biochemical Engineering, Rutgers University
The biological importance of carbohydrates (or glycans) can be better understood and exploited by creating a robust toolkit. Here, we highlight recent advances in glycoengineered microbial strains/plasmids and enzymes development for in vivo conversion of azido sugars to designer glycans (e.g., Human Milk Oligosaccharides). We discuss how such a toolkit can be used for efficient recombinant protein production as well as directed evolution of carbohydrate-active enzymes (e.g., glycosynthases).
Reference: Bandi et al. 2021, ACS Synthetic Biology, 10:4, 682–689. https://doi.org/10.1021/acssynbio.0c00449.